You want to use enough solution to soak into all areas of the urine stain. You must be careful not to let the solution sink into the subflooring. [2] X Trustworthy Source The Humane Society of the United States National organization devoted to the promotion of animal welfare Go to source

Put a laundry bucket over the area while the baking soda is cleaning the area. This will prevent your pet or any small children from messing with the area.

Apply the cleaner according to package’s directions. Repeat as necessary. Read the safety measures to ensure the product doesn’t contain hazardous materials.

You can make this opening a long slot, rather than a square, so that it’s easier to leave open permanently. For example, you could cut a slot that’s 1 inch (2. 5 cm) wide and 12 feet (3. 7 m) long, placing it along the length of the stall. If possible, place the slot near the ceiling of the stable, which allows the freshest air to circulate into the stall. In high concentrations, ammonia can cause respiratory damage and even death. [6] X Research source

Use tightly interlocking or seamless stall mats to keep the urine from seeping into the floor underneath the bedding. [8] X Research source

If the horse is in and out of the stall and you have mats, cover the mats with about 2 inches of bedding.

Ammonium hydroxide(ammonia dissolved in water) is a common cleaning agent. You can dilute the spill with water and use the mixture as an impromptu cleaner. You’ll need to add a higher ratio of water to ammonia, so if you spilled too much, use the dry mixture. This combination of dry ingredients is a safe way of containing and disposing large amounts of liquid ammonia.

Search for ammonia neutralizers. Some brands develop a mixture of acids to be used to neutralize ammonia. Hydrochloric acid is found in some tile and toilet bowl cleaners, as well as stain removers. You can also buy pure hydrochloric acid online. Sulfuric acid is found in toilet bowl cleaners and drain de-cloggers. You can buy it online as well. This acid is highly corrosive, so read the safety instructions on the bottle carefully and wear gloves when handling it.

Throw this mixture directly into your outdoor trash bin if you don’t have access to a fume hood.

If you added too much, the neutralizer will still do the job, but may need more applications of water to the spill area. [13] X Research source Stir the mixture for about thirty seconds.

On the second cleaning, use water and a surface cleaner to ensure a thorough cleaning of the area. Never use bleach to clean ammonia because the combined chemicals will create a poisonous gas harmful to people. [14] X Research source